The bulk of the weight of the bike should be resting on the floor, so this will work for heavy aluminum mountain bikes (with modifications) as well as lighter road bikes.
STL file included will allow for a 27mm tire width, and 50mm difference in tire radii.
Before printing, edit your measurements in the included SCAD file or the customizer to fit your bike. INNER_WIDTH should be your tire width, and TIRE_SIZE should be the distance between the outer and inner diameters of your tire (in mm). INNER_WIDTH should be as tight as is convenient to maximize strength.
Infill depends on weight of bike. The less the tire can rotate within the bracket the less torsion (and consequently less infill) is necessary.